Pin1 Antibody

Chicken products cannot be exported to Canada.

Pin1 (peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is a small enzyme which is unique amongst all peptidyl-prolyl isomerases (PPIases) as it interacts with phosphorylated serine/threonine residues followed by a proline (pS/T-P). Localized in nucleus and cytoplasm, Pin1 interacts with STIL, KIF20B, NEK6, PRKX, BTK, DAPK1, ATCAY and phosphorylated RAF1. It specifically isomerizes pS/T-P peptide bonds of its target proteins and considering the number of phosphoproteins with pS/T-P motifs in cells, it is not surprising that Pin1 has such profound influence on multiple biological processes. ATM/ATR facilitates its phosphorylation upon DNA damage and DAPK1 mediated phosphorylation at Ser-71 leads to inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation. Pin1 is regarded as regulatory timer of cell signaling processes and it is implicated in transcription and enzymatic activity, protein phosphorylation, stability, processing, localization and interaction, cellular growth/proliferation, apoptosis and immune responses. Moreover, Pin1's deregulation contributes to many human pathologies such as aging, cancer, neurological disorders including Alzheimer's disease, autoimmune and inflammatory diseases.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-P.

Showing Publications 1 - 2 of 2.

Publications using NBP1-05424	Applications	Species
Yoon WJ, Islam R, Cho YD et al. Pin1 Plays a Critical Role as a Molecular Switch in Canonical BMP Signaling. J. Cell. Physiol. 2014-09-04 [PMID: 25187260] (ICC/IF, Mouse)	ICC/IF	Mouse
Islam R, Bae HS, Yoon WJ et al. Pin1 Regulates Osteoclast Fusion through Suppression of the Master Regulator of Cell Fusion DC-STAMP J. Cell. Physiol. 2014-06-02 [PMID: 24891219] (IHC-P, ICC/IF, Mouse) Details: Pin1 antibody used for IHC-P and ICC-IF in Pin1 +/- and Pin1 -/- mice. For IHC-P, antigen retreival provided efficient signal (boiled the tissues in Tris-EDTA buffer pH 9.2 for 20 min/60C) and biotinylated Mouse on Mouse (MOM) anti-Mouse Ig reagent was used for blocking the non-specific signal, followed by probing with primary and staining development with Alexa555-red labelled secondary antibody (Figure 4D). In ICC-IF on mouse's cultured osteoclasts, Triton-X (1%) was used for permeabilization and staining was developed with Alexa555-red labelled secondary antibody (Figure 4C).	IHC-P, ICC/IF	Mouse

Array NBP1-05424

• Pin1 Antibodies
• Pin1 Lysate
• Pin1 Proteins
• Pin1 Proteins and Enzyme