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PIM3 Antibody (4A9)

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Sandwich ELISA: PIM3 Antibody (4A9) [H00415116-M10] - Detection limit for recombinant GST tagged PIM3 is 0.03 ng/ml as a capture antibody.
Sandwich ELISA: PIM3 Antibody (4A9) [H00415116-M10] - Detection limit for recombinant GST tagged PIM3 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PIM3 Antibody (4A9) Summary

Immunogen
PIM3 (NP_001001852.1, 242 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSSSESL
Specificity
PIM3 - pim-3 oncogene (4A9)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIM3
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIM3 Antibody (4A9)

  • EC 2.7.11.1
  • pim-3 oncogene
  • PIM3
  • pim-3
  • serine/threonine kinase Pim-3
  • serine/threonine-protein kinase pim-3

Background

PIM3 belongs to a family of protooncogenes that encode serine/threonine protein kinases (Mikkers et al., 2004 [PubMed 15199164]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PIM3 Antibody (4A9)
H00415116-M10
PIM3 Antibody (4A9)
Species: Hu
Applications: WB, ELISA

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PIM3 Antibody (H00415116-M10). (Showing 1 - 1 of 1 FAQ).

  1. Does above pim3 antibody has cross reaction with pim 1 & 2 proteins?
    • I ran an alignment on the sequence and I would not predict it to react, but we have not tested for reactivity directly so I cannot guarantee that. Based on the sequence information however, I think it is safe to assume the antibody won't detect PIM1 and PIM2. Here is the sequence: DIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSSSESL. I hope you find this information useful.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PIM3
Entrez
Uniprot