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PHF8 Antibody (SR1157)

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Western Blot: PHF8 Antibody (SR1157) [NBP3-21826] - Western blot analysis using NBP3-21826 in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SR1157
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PHF8 Antibody (SR1157) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human PHF8 (Uniprot #: Q9UPP1)
Specificity
Detects endogenous levels of total PHF8
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PHF8
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation 1:70
  • Western Blot 1:500-1:2000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PHF8 Antibody (SR1157)

  • EC 1.14.11.27
  • histone lysine demethylase PHF8
  • jumonji C domain-containing histone demethylase 1F
  • KIAA1111
  • KIAA1111ZNF422JHDM1F
  • MRXSSD
  • PHD finger protein 8DKFZp686E0868
  • PHF8
  • ZNF422

Background

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PHF8