Novus Biologicals products are now on bio-techne.com

Persephin Antibody

Images

 
There are currently no images for Persephin Antibody (AF2388).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Persephin Antibody Summary

Immunogen
E. coli-derived recombinant human Persephin
Ala61-Gly156
Accession # O60542
Specificity
Detects human Persephin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Persephin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSPN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Persephin Antibody

  • Persephin
  • PSP
  • PSPN

Background

Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38-46% amino acid (aa) identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 156 aa, 10-12 kDa mature protein contains a signal sequence, a pro-domain and a 96 aa mature sequence with several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature human Persephin shares 81% and 80%, 89%, and 87% amino acid sequence identity with mouse, rat, bovine and canine Persephin, respectively. Like other GDNF family members, Persephin acts through engagement of GRF alpha 4, a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, Persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the Persephin gene results in mice that are morphologically normal but have more damage and less effective repair after central nervous system insult that stimulates a stroke. Microinjection of Persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of Persephin are detrimental (7).

  1. Milbrandt, J. et al. (1998) Neuron 20:245.
  2. Lindahl, M. et al. (2001) J. Biol. Chem. 276:9344.
  3. Lindahl, M. et al. (2000) Mol. Cell. Neurosci. 15:522.
  4. Akerud, P. et al. (2002) Mol. Cell. Neurosci. 21:205.
  5. Enokido, Y. et al. (1998) Current Biol. 8:1019.
  6. Yang, J. et al. (2004) FEBS Lett. 569:267.
  7. Tomac, A.C. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9521.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-25162
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
212-GD
Species: Hu
Applications: Bind, BA
1297-NE/CF
Species: Hu
Applications: Bind, BA
1085-AR
Species: Mu
Applications: Bind, BA
AF482
Species: Mu
Applications: IHC, WB
NBP2-37493
Species: Hu, Mu, Po
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF1445
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
MAB4937
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
NBP1-80670
Species: Hu
Applications: IHC, IHC-P, WB
267-N3
Species: Hu
Applications: BA
7954-GM/CF
Species: Hu
Applications: BA
NBP2-15365
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, S-ELISA, WB
NBP2-42388
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF6590
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
NB100-1519
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
NBP1-82452
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AF2388
Species: Hu
Applications: WB

Publications for Persephin Antibody (AF2388) (0)

There are no publications for Persephin Antibody (AF2388).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Persephin Antibody (AF2388) (0)

There are no reviews for Persephin Antibody (AF2388). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Persephin Antibody (AF2388) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Persephin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PSPN
Uniprot