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Rat PCK1 ELISA Kit (Colorimetric)

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ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Samples were spiked with high concentrations of Rat PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Rat PCK1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PCK1 in samples. No significant cross-reactivity or interference between Rat PCK1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.2%
Intra-Assay
CV% < 5.08%
Spike Recovery
89-108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PCK1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat PCK1 ELISA Kit (Colorimetric)

  • EC 4.1.1.32
  • MGC22652
  • PCK1
  • PEPCK1
  • PEPCKC
  • PEPCK-C
  • PEPCK-CPEP carboxykinase
  • phosphoenolpyruvate carboxykinase 1 (soluble)
  • phosphoenolpyruvate carboxykinase, cytosolic [GTP]
  • phosphoenolpyruvate carboxykinase, cytosolic
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

The PCK1 gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expressio

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PCK1