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PBX2 Antibody (PCRP-PBX2-1C4)

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Flow Cytometry: PBX2 Antibody (PCRP-PBX2-1C4) [NBP3-13938] - Flow cytometric analysis of PFA-fixed HeLa cells. PBX2 antibody (PCRP-PBX2-1C4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Flow Cytometry: PBX2 Antibody (PCRP-PBX2-1C4) [NBP3-13938] - Flow cytometric analysis of PFA-fixed HeLa cells. PBX2 antibody (PCRP-PBX2-1C4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: PBX2 Antibody (PCRP-PBX2-1C4) [NBP3-13938] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PBX2 Antibody (PCRP-PBX2-1C4).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IP, MA
Clone
PCRP-PBX2-1C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14127AF350
Alexa Fluor 405
NBP3-14127AF405
Alexa Fluor 488
NBP3-14127AF488
Alexa Fluor 532
NBP3-14127AF532
Alexa Fluor 594
NBP3-14127AF594
Alexa Fluor 647
NBP3-14127AF647
Alexa Fluor 700
NBP3-14127AF700
Alexa Fluor 750
NBP3-14127AF750
Allophycocyanin
NBP3-14127APC
Biotin
NBP3-14127B
CoraFluor 1
NBP3-14127CL1
DyLight 350
NBP3-14127UV
DyLight 405
NBP3-14127V
DyLight 488
NBP3-14127G
DyLight 550
NBP3-14127R
DyLight 594
NBP3-14127DL594
DyLight 650
NBP3-14127C
DyLight 680
NBP3-14127FR
DyLight 755
NBP3-14127IR
FITC
NBP3-14127F
HRP
NBP3-14127H
Janelia Fluor 525
NBP3-14127JF525
Janelia Fluor 549
NBP3-14127JF549
Janelia Fluor 646
NBP3-14127JF646
Janelia Fluor 669
NBP3-14127JF669
PE
NBP3-14127PE
PerCP
NBP3-14127PCP
mFluor Violet 450 SE
NBP3-14127MFV450
mFluor Violet 500 SE
NBP3-14127MFV500
mFluor Violet 610 SE
NBP3-14127MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14127
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PBX2 Antibody (PCRP-PBX2-1C4) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14127)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human PBX2 protein (Uniprot: P40425)
Localization
Nucleus
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PBX2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PBX2 Antibody (PCRP-PBX2-1C4)

  • G17Homeobox protein PBX2
  • HOX12homeobox 12
  • PBX2MHC
  • pre-B-cell leukemia homeobox 2
  • pre-B-cell leukemia transcription factor 2
  • Protein G17

Background

PBX2 encodes a ubiquitously expressed member of the TALE/PBX homeobox family. It was identified by its similarity to a homeobox gene which is involved in t(1;19) translocation in acute pre-B-cell leukemias. This protein is a transcriptional activator which binds to the TLX1 promoter. The gene is located within the major histocompatibility complex (MHC) on chromosome 6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PBX2
Uniprot