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Pannexin-1 Antibody

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Genetic Strategies: Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody [NBP1-92241] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human small intestine shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Pannexin-1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PANX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
Pannexin-1 Protein (NBP1-92241PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pannexin-1 Antibody

  • Innexin
  • MGC21309
  • MRS1
  • MRS1innexin
  • pannexin 1
  • Pannexin1
  • Pannexin-1
  • PANX1
  • PX1
  • UNQ2529

Background

The protein encoded by the Pannexin 1 gene belongs to the innexin family. Innexin family members are the structural components ofgap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressedin various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination withpannexin 2 may form cell type-specific gap junctions with distinct properties. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pannexin-1 Antibody (NBP1-92241) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PANX1