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PAK1/2/3 [p Ser144] Antibody (SR2102)

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Immunohistochemistry: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] - Immunohistochemical analysis of paraffin-embedded mouse brain, using NBP3-21523.
Immunohistochemistry: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] - Immunohistochemical analysis of paraffin-embedded mouse brain, using NBP3-21523.
Western Blot: PAK1/2/3 [p Ser 144] Antibody (SR2102) [NBP3-21523] - Western blot analysis using NBP3-21523 in HeLa Cell lysate treated with lambda phosphatase.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
SR2102
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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PAK1/2/3 [p Ser144] Antibody (SR2102) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-PAK1/2/3 [Ser144/Ser141/Ser139] (Uniprot #: O75914, Q13153, Q13177)
Modification
p Ser144
Specificity
Detects endogenous levels of total phospho-PAK1/2/3 [Ser144/Ser141/Ser139]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PAK1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-1:10000
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PAK1/2/3 [p Ser144] Antibody (SR2102)

  • alpha-PAK
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC130000
  • MGC130001
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • p21-activated kinase 1
  • p65-PAK
  • PAK-1
  • PAKalpha
  • serine/threonine-protein kinase PAK 1
  • STE20 homolog, yeast

Background

In mammals, there are several identified isoforms of p21-activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAK1