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P2X4 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining in human placenta and skeletal muscle tissues . Corresponding P2RX4 RNA-seq data are presented for the same tissues.
Western Blot: P2X4 Antibody [NBP1-92239] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: P2X4 Antibody [NBP1-92239] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human cerebral cortex shows positivity in neurons.
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP1-92239] - Staining of human small intestine shows positivity in luminal membrane in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

P2X4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YCMKKRLYYREKKYKYVEDYEQGLASELDQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P2RX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
P2X4 Protein (NBP1-92239PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for P2X4 Antibody

  • ATP receptor
  • ATP-gated cation channel protein
  • P2RX4
  • P2X purinoceptor 4
  • P2X4
  • P2X4P2X receptor, subunit 4
  • P2X4R
  • purinergic receptor P2X, ligand-gated ion channel, 4
  • Purinergic receptor
  • purinoceptor P2X4

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol P2RX4