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Human p130Cas/BCAR1 ELISA Kit (Colorimetric)

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ELISA: Human p130Cas/BCAR1 ELISA Kit (Colorimetric) [NBP2-69941] - Samples were spiked with high concentrations of Human p130Cas/BCAR1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human p130Cas/BCAR1 ELISA Kit (Colorimetric) [NBP2-69941] - Samples were spiked with high concentrations of Human p130Cas/BCAR1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human p130Cas/BCAR1 ELISA Kit (Colorimetric) [NBP2-69941] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Human p130Cas/BCAR1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human BCAR1 in samples. No significant cross-reactivity or interference between Human BCAR1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.68%
Intra-Assay
CV% < 5.02%
Spike Recovery
83-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BCAR1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human p130Cas/BCAR1 ELISA Kit (Colorimetric)

  • BCAR1
  • breast cancer estrogen resistance 1
  • Cas scaffolding protein family member 1
  • CAS1
  • CASFLJ45059
  • CASS1
  • CASS1CRKASbreast cancer estrogen resistance protein 1
  • CRKAS
  • Crk-associated substrate p130Cas
  • CRK-associated substrate
  • FLJ12176
  • p130Cas

Background

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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NBP2-69941
Human p130Cas/BCAR1 ELISA Kit (Colorimetric)
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Product PDFs

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  Human p130Cas/BCAR1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol BCAR1