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Osteopontin/OPN Antibody Blocking Peptide

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Summary
Reactivity HuSpecies Glossary
Applications AC

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Osteopontin/OPN Antibody Blocking Peptide Summary

Description
A Human Osteopontin/OPN antibody blocking peptide

Source: Synthetic (Accession #: P10451)

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Source
Synthetic
Protein/Peptide Type
Antibody Blocking Peptide
Gene
SPP1
Purity
>95%

Applications/Dilutions

Dilutions
  • Antibody Competition
Application Notes
Control peptide should be used at 1.0 ug per 1.0 ul of antiserum per assay. MW 1879 daltons. Osteopontin peptide has been tested in SDS-page.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Purity
>95%

Alternate Names for Osteopontin/OPN Antibody Blocking Peptide

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SPP1
Uniprot