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Ornithine Carbamoyltransferase Antibody

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Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-54934] - Reccomended Titration: 2.5 ug/ml Positive Control: HepG2 cell lysate
Immunohistochemistry: Ornithine Carbamoyltransferase Antibody [NBP1-54934] - Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal villas Antibody Concentration: 4.0 - 8.0 ug/ml ...read more
Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-54934] - Sample Tissue: HepG2, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1.25ug/mL, Peptide ...read more
Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-54934] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Ornithine Carbamoyltransferase Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to OTC(ornithine carbamoyltransferase) The peptide sequence was selected from the N terminal of OTC. Peptide sequence AFRNGHNFMVRNFRCGQPLQNKVQLKGRDLLTLKNFTGEEIKYMLWLSAD. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OTC
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Ornithine Carbamoyltransferase Antibody

  • EC 2.1.3
  • EC 2.1.3.3
  • MGC129967
  • MGC129968
  • MGC138856
  • OCTD
  • Ornithine Carbamoyltransferase
  • ornithine carbamoyltransferase, mitochondrial
  • Ornithine Transcarbamylase
  • OTC
  • OTCase

Background

OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol OTC
Uniprot