![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47862-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
| Reactivity | Hu, Bv(-), Mu(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | 364-5 + NCL/902 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44613). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 ) |
| Localization | Nucleoli |
| Marker | Marker of Human Cells |
| Specificity | Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. |
| Isotype | IgG1 Kappa/IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NCL |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 76 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nucleolin Antibody (NBP2-47862)Find related products by research area.
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Nucleolin: A Multifaceted Nucleolar Phosphoprotein Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n... Read full blog post. |
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Nucleolin Antibodies: Knowing When it's Time to Split Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ... Read full blog post. |
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Nucleolin: To the Nucleus and Beyond! Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres... Read full blog post. |
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| Gene Symbol | NCL |
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47862-img0008.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)")
![Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47862-img0010.jpg "Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47862-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Colon Carcinoma stained with AF488 Conjugate of Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47862-img0002.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Colon Carcinoma stained with AF488 Conjugate of Nucleolin Monoclonal Antibody (364-5 + NCL/902).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47862-img0005.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47862-img0006.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).")
![Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47862-img0004.jpg "Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) - Azide and BSA Free [NBP2-47862] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
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