![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0005.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, PoSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-SIPTDNQIKARK corresponding to C-Terminus according to NP_000894.1, NP_001020604.1, NP_001020605.1. |
| Specificity | This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | NQO1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_058696.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NQO-1 Antibody (NB100-1005)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1005-img0001.jpg "Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NB100-1005-img0002.jpg "Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0003.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0004.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence")
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