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Notch-3 Antibody

Images

 
Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Placenta. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Notch-3 Antibody [NB100-2414] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Notch-3 Antibody [NB100-2414] - Staining of paraffin embedded Human Hippocampus. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Notch-3 Antibody Summary

Immunogen
Peptide with sequence C-QLGPQPEVTPKRQ corresponding to C-Terminus according to NP_000426.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOTCH3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:16000
Application Notes
Notch-3 Antibody validated for IP from a verified customer review.
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2414 in the following applications:

Publications
Read Publication using NB100-2414.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Notch-3 Antibody

  • CADASIL
  • CASILneurogenic locus notch homolog protein 3
  • Notch (Drosophila) homolog 3
  • notch 3Notch homolog 3 (Drosophila)
  • Notch homolog 3
  • Notch3
  • Notch-3

Background

The NOTCH3 gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Notch-3 Antibody (NB100-2414)(1)


Review for Notch-3 Antibody (NB100-2414) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2414:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IP Human 09/15/2009
View

Summary

ApplicationImmunoprecipitation
Sample Testedhuman lung cancer
SpeciesHuman
Lot080806

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FAQs for Notch-3 Antibody (NB100-2414) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Verified Customer
09/15/2009
Application: IP
Species: Human

Bioinformatics

Gene Symbol NOTCH3
Entrez
Uniprot