Novus Biologicals products are now on bio-techne.com

Notch-2 Antibody (602845) [Unconjugated]

Images

 
NTera‑2 human testicular embryonic carcinoma cell line was stained with Human Notch‑2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more
Raji Burkit lymphoma human cell line was stained with Human Notch-2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
602845
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Notch-2 Antibody (602845) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Notch-2
Leu26-Gln530
Accession # Q04721
Specificity
Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOTCH2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB37351 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-2 Antibody (602845) [Unconjugated]

  • AGS2
  • hN2
  • neurogenic locus notch homolog protein 2
  • Notch (Drosophila) homolog 2
  • notch 2Notch homolog 2 (Drosophila)
  • Notch homolog 2
  • Notch2
  • Notch-2

Background

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-78486
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
AF3847
Species: Hu
Applications: ICC, WB
H00004854-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-47791
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
NBP1-33427
Species: Hu
Applications: ChIP, ICC/IF, WB
1277-JG
Species: Hu
Applications: BA
AF5026
Species: Mu, Rt
Applications: IHC, WB
1726-JG
Species: Hu
Applications: BA
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-16818
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP1-89680
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-27174
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
AF1389
Species: Mu
Applications: ICC, IHC, WB
NB100-74510
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP3-03905
Species: Hu, Mu, Rt
Applications: WB
AF2567
Species: Mu
Applications: IHC, WB
MAB37351
Species: Hu
Applications: Flow, CyTOF-ready

Publications for Notch-2 Antibody (MAB37351)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: In-Cell Western.


Filter By Application
In-Cell Western
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Notch-2 Antibody (MAB37351) (0)

There are no reviews for Notch-2 Antibody (MAB37351). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-2 Antibody (MAB37351) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Notch-2 Antibody (602845) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol NOTCH2
Uniprot