![Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).](http://images.novusbio.com/fullsize/NMDA-receptor-N1-Antibody-Western-Blot-NB300-114-img0004.jpg "Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).")
| Reactivity | Mu, Rt, Hu, Ca, Ch, Pm, XpSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | The antigen is a peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage). |
| Immunogen | Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH. Accession # P35439 |
| Specificity | Specific for endogenous levels of the ~120 kDa NMDA R, NR1 Subunit splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert. |
| Predicted Species | Human (100%), Chicken (100%), Canine (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GRIN1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | NB 300-114 can be used in Western blot where a band is seen at ~ 100 kDa representing the NMDAR1 N1 splice variant. |
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| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Antigen Affinity-purified |
| Reconstitution Instructions | Reconstitute with 50 uL PBS to desired concentration. |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Flow | Mouse | 01/31/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NMDA R, NR1 Subunit Antibody (NB300-114)Find related products by research area.
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![Flow Cytometry: NMDA receptor N1 Antibody [NB300-114] - CD4+ Lymphocytes expressing NMDAR1. Image provided by verified customer.](http://images.novusbio.com/fullsize/NMDA-receptor-N1-Antibody-Flow-Cytometry-NB300-114-img0002.jpg "Flow Cytometry: NMDA receptor N1 Antibody [NB300-114] - CD4+ Lymphocytes expressing NMDAR1. Image provided by verified customer.")
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