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NKX2.5 Antibody (259416)

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NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
259416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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NKX2.5 Antibody (259416) Summary

Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Specificity
Detects human NKX-2.5 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB2444 in the following applications:

  • ICC
    3 publications
  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.5 Antibody (259416)

  • cardiac-specific homeo box
  • cardiac-specific homeobox 1
  • Cardiac-specific homeobox
  • CHNG5
  • CSX1
  • CSXFLJ97195
  • FLJ52202
  • FLJ99536
  • Homeobox protein CSX
  • Homeobox protein NK-2 homolog E
  • homeobox protein Nkx-2.5
  • NK2 transcription factor related, locus 5 (Drosophila)
  • NKX2.5
  • NKX2.5FLJ97166
  • NKX2-5
  • NKX2E
  • NKX2EFLJ97197
  • NKX4-1
  • tinman paralog

Background

NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX2.5 Antibody (MAB2444)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC, IHC.


Filter By Application
ICC
(3)
IHC
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB2444 Applications Species
Wei Feng, Hannah Schriever, Shan Jiang, Abha Bais, Haodi Wu, Dennis Kostka et al. Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency Communications Biology 2022-04-29 [PMID: 35488063]
Feng Liu, Dandan Long, Wenjun Huang, Wanling Peng, Huan Lan, Yafei Zhou et al. The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSC International Journal of Stem Cells 2022-08-30 [PMID: 35220280]
L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020-08-11;0(0):. 2020-08-11 [PMID: 32783886] (IHC, ICC, Human) IHC, ICC Human
T Colunga, M Hayworth, S Kre beta , DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 2019-03-05;26(10):2566-2579.e10. 3/5/2019 [PMID: 30840882]
S Ranjbarvaz, S Park, NB Nguyen, WB Gilmore, P Zhao, R Ardehali Generation of Nkx2-5/CreER Transgenic Mice for Inducible Cre Expression in Developing Hearts Genesis, 2017-06-15;0(0):. 6/15/2017 [PMID: 28589709]
IA Song, AY Oh, JH Kim, YM Choi, YT Jeon, JH Ryu, JW Hwang The involvement of protein kinase C-? in isoflurane induced preconditioning of human embryonic stem cell--derived Nkx2.5(+) cardiac progenitor cells BMC Anesthesiol, 2016-02-20;16(0):13. 2016-02-20 [PMID: 26897636] (ICC, Human) ICC Human
Gregoire S, Karra R, Passer D, Deutsch M, Krane M, Feistritzer R, Sturzu A, Domian I, Saga Y, Wu S Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation. Circ Res, 2013-01-10;112(6):900-10. 2013-01-10 [PMID: 23307821] (IHC, Mouse) IHC Mouse
Sonia Stefanovic, Nesrine Abboud, Stéphanie Désilets, David Nury, Chad Cowan, Michel Pucéat Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate Journal of Cell Biology 2009-09-07 [PMID: 19736317]
Jumabay M, Matsumoto T, Yokoyama S, Kano K, Kusumi Y, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H, Fukuda N Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J. Mol. Cell. Cardiol., 2009-08-15;47(5):565-75. 2009-08-15 [PMID: 19686758] (ICC, Rat) ICC Rat

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Bioinformatics

Gene Symbol NKX2-5
Uniprot