Home » NKX2.5 » NKX2.5 Antibodies » NKX2.5 Antibody (259415) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

NKX2.5 Antibody (259415) [Unconjugated]

 
 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
259415
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB24441-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

NKX2.5 Antibody (259415) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Specificity
Detects endogenous human NKX2.5 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.5 Antibody (259415) [Unconjugated]

  • cardiac-specific homeo box
  • cardiac-specific homeobox 1
  • Cardiac-specific homeobox
  • CHNG5
  • CSX1
  • CSXFLJ97195
  • FLJ52202
  • FLJ99536
  • Homeobox protein CSX
  • Homeobox protein NK-2 homolog E
  • homeobox protein Nkx-2.5
  • NK2 transcription factor related, locus 5 (Drosophila)
  • NKX2.5
  • NKX2.5FLJ97166
  • NKX2-5
  • NKX2E
  • NKX2EFLJ97197
  • NKX4-1
  • tinman paralog

Background

NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart. NKX2.5 protein contains a central single DNA binding domain flanked by two Ala/Pro rich domains. Sumoylation of NKX2.5 at Lys-51 enhances its DNA binding and transcriptional activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GATA-4 Antibody -
BSA Free
NBP2-24585
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
Western Blot GATA-4 Antibody - BSA Free
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
GATA-4 Antibody -
BSA Free
NBP2-24585
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
Western Blot GATA-4 Antibody - BSA Free
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
MEF2C Antibody
(OTI1H5)
NBP2-00493
Flow Cytometry MEF2C Antibody (OTI1H5)
Flow Cytometry MEF2C Antibody (OTI1H5)
Flow Cytometry MEF2C Antibody (OTI1H5)
(3 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TBX5 Antibody
NBP1-83237
Immunohistochemistry-Paraffin TBX5 Antibody
Immunohistochemistry-Paraffin TBX5 Antibody
Immunohistochemistry-Paraffin TBX5 Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
preproANP Antibody
NBP2-14873
Immunohistochemistry-Paraffin preproANP Antibody
Immunohistochemistry-Paraffin preproANP Antibody
Western Blot preproANP Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(522 Publications)
Species: Hu
Applications: BA, BA
Serum Response
Factor SRF Antibody ( ...
NBP3-13785
Flow Cytometry Serum Response Factor SRF Antibody (PCRP-SRF-1F7)
Immunocytochemistry/ Immunofluorescence Serum Response Factor SRF Antibody (PCRP-SRF-1F7)
Immunohistochemistry-Paraffin Serum Response Factor SRF Antibody (PCRP-SRF-1F7)
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(190 Publications)
Species: Hu, Mu, Rt
Applications: BA
Islet-1 Antibody
[Unconjugated]
AF1837
Simple Western Islet-1 Antibody [Unconjugated]
Western Blot Islet-1 Antibody [Unconjugated]
Immunocytochemistry Islet-1 Antibody [Unconjugated]
(37 Publications)
Species: Hu
Applications: ICC, IHC, Simple Western, WB
HESX1 Antibody
NBP1-89090
Immunohistochemistry-Paraffin HESX1 Antibody
Immunohistochemistry-Paraffin HESX1 Antibody
Immunohistochemistry-Paraffin HESX1 Antibody
Species: Hu
Applications: IHC, IHC-P
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(77 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
HAND2 Antibody
[Unconjugated]
AF3876
Immunohistochemistry HAND2 Antibody [Unconjugated]
(5 Publications)
Species: Hu, Mu
Applications: IHC, WB
TBX1 Antibody
(OTI1C2)
NBP2-46076
Western Blot TBX1 Antibody (OTI1C2)
Immunohistochemistry-Paraffin TBX1 Antibody (OTI1C2)
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
TTF-1 / NKX2-1
Antibody (8G7G3/1 + N ...
NBP2-44501
Immunohistochemistry TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)
(1 Review)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IF, IHC, IHC-P
Connexin 40/GJA5
Antibody
NBP3-41541
Western Blot Connexin 40/GJA5 Antibody
Immunohistochemistry-Paraffin Connexin 40/GJA5 Antibody
Immunohistochemistry-Paraffin Connexin 40/GJA5 Antibody
Species: Rt
Applications: ICC/IF, IHC, WB
Myocardin Antibody
(355521) [Unconjugate ...
MAB4028
Western Blot Myocardin Antibody (355521) [Unconjugated]
Western Blot Myocardin Antibody (355521) [Unconjugated]
Western Blot Myocardin Antibody (355521) [Unconjugated]
(15 Publications)
Species: Hu, Mu
Applications: WB

Publications for NKX2.5 Antibody (MAB24441) (0)

There are no publications for NKX2.5 Antibody (MAB24441).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKX2.5 Antibody (MAB24441) (0)

There are no reviews for NKX2.5 Antibody (MAB24441). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKX2.5 Antibody (MAB24441) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NKX2.5 Products

Blogs on NKX2.5

There are no specific blogs for NKX2.5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NKX2.5 Antibody (259415) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol NKX2-5
Uniprot