![Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-288202412442980.jpg "Western Blot: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Western blot analysis of NKCC2/SLC12A1 on different lysates with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/1,000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Tissue lysates at 30 ug/Lane. Cell lysates at 20 ug/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32951) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JE64-26 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human NKCC2/SLC12A1 aa 593-813 / 1,099. (Uniprot: Q13621) |
| Localization | Cell membrane |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SLC12A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 121 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SLC12A1 |
![Immunohistochemistry: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32951) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-298202414333656.jpg "Immunohistochemistry: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32951) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunocytochemistry analysis of K562 cells labeling NKCC2/SLC12A1 with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde and 80% methanol for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32951_rabbit-nkcc2-slc12a1-mab-je64-26-2920241192380.jpg "Immunocytochemistry/ Immunofluorescence: NKCC2/SLC12A1 Antibody (JE64-26) [NBP3-32951] - Immunocytochemistry analysis of K562 cells labeling NKCC2/SLC12A1 with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde and 80% methanol for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NKCC2/SLC12A1 antibody (NBP3-32951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")