![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143] - Analysis of Nicotinic Acetylcholine Receptor alpha 4 in Rat Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-Western-Blot-NBP1-00143-img0003.jpg "Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143] - Analysis of Nicotinic Acetylcholine Receptor alpha 4 in Rat Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Rt, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IP (-) |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1. |
| Epitope | internal region |
| Specificity | This antibody is expected NOT to cross-react with the similar alpha 2 subunit. |
| Predicted Species | Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CHRNA4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-00143 | Applications | Species |
|---|---|---|
| Schwarz J, Schwarz SC, Dorigo O et al. Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons. FASEB J 2006-05-01 [PMID: 16675851] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (NBP1-00143)Find related products by research area.
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| Gene Symbol | CHRNA4 |
| Entrez | |
| Uniprot |
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