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Kv7.2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Kv7.2 Antibody [NBP2-38820] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: Kv7.2 Antibody [NBP2-38820] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry: Kv7.2 Antibody [NBP2-38820] - Staining of human hippocampus shows distinct nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: Kv7.2 Antibody [NBP2-38820] - Staining of human cerebral cortex shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Kv7.2 Antibody [NBP2-38820] - Staining in human cerebral cortex and pancreas tissues using anti-KCNQ2 antibody. Corresponding KCNQ2 RNA-seq data are presented ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Kv7.2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RLGKVEKQVLSMEKKLDFLVNIYMQRMGIPPTETEAYFGAKEPEPAPPYHSPEDSREHVDRHGCIVKIVRSSSST
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KCNQ2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Kv7.2 Protein (NBP2-38820PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Kv7.2 Antibody

  • BFNC
  • BFNS1
  • EBN
  • EBN1
  • EIEE7
  • ENB1
  • HNSPC
  • KCNA11
  • KCNQ2
  • KQT-like 2
  • Kv7.2
  • KVEBN1
  • neuroblastoma-specific potassium channel protein
  • Neuroblastoma-specific potassium channel subunit alpha KvLQT2
  • potassium voltage-gated channel subfamily KQT member 2
  • potassium voltage-gated channel, KQT-like subfamily, member 2
  • Voltage-gated potassium channel subunit Kv7.2

Background

KCNQs are members of the voltage dependent non inactivating potassium channel family. Currently there are five known KCNQs (KCNQ1 to 5) found in the central nervous system and KCNQ2 and 3 have demonstrated their importance in M current activation. Studies have shown that KCNQ2 and KCNQ3 form heteromultimers that, when formed, substantially increase the M current. Inhibition of M current controls neuron excitability throughout the nervous system as well as the responsiveness to synaptic inputs. Genetic mutations in these proteins have been linked to disorders such as benign familial neonatal convulsions (BFNC), deafness, neuropathic pain and epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KCNQ2
Uniprot