Home » NHERF-1 » NHERF-1 Proteins » Recombinant Human NHERF-1 GST (N-Term) Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Human NHERF-1 GST (N-Term) Protein

 Submit a Review

Images

 
(
Enlarge)
12.5% SDS-PAGE Stained with Coomassie Blue.
12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human NHERF-1 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-358 of Human SLC9A3R1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL
Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
NHERF1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
65.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human NHERF-1 GST (N-Term) Protein

  • EBP50NHERF-1
  • ezrin-radixin-moesin binding phosphoprotein-50
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF1
  • Na+/H+ exchange regulatory co-factor
  • NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • NHERFNPHLOP2
  • Regulatory cofactor of Na(+)/H(+) exchanger
  • sodium/hydrogen exchanger regulatory factor 1
  • Sodium-hydrogen exchanger regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Solute carrier family 9 isoform A3 regulatory factor 1

Background

Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NHERF-2 Antibody
NB100-2450
Western Blot NHERF-2 Antibody
Immunohistochemistry-Paraffin NHERF-2 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus
Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus
Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus
(10 Reviews)
(76 Publications)
Species: Hu
Applications: IHC, IP, WB
Ezrin Antibody
NBP2-16396
Immunohistochemistry-Paraffin Ezrin Antibody
Immunohistochemistry-Paraffin Ezrin Antibody
Western Blot Ezrin Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC-WhMt, IHC, IHC-P, WB
NHE3/SLC9A3
Antibody
NBP1-82574
Immunohistochemistry-Paraffin NHE3/SLC9A3 Antibody
Immunohistochemistry-Paraffin NHE3/SLC9A3 Antibody
Immunohistochemistry-Paraffin NHE3/SLC9A3 Antibody
(31 Publications)
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, PAGE, WB
PTH Antibody
(918462) [Unconjugate
MAB7665
Immunohistochemistry PTH Antibody (918462) [Unconjugated]
Western Blot PTH Antibody (918462) [Unconjugated]
Species: Hu
Applications: IHC, WB
PDZK1 Antibody
NB400-149
Western Blot PDZK1 Antibody
Immunocytochemistry/ Immunofluorescence PDZK1 Antibody
Immunohistochemistry PDZK1 Antibody
(5 Publications)
Species: Bv, Hu, Mu, Rt(-)
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Tight Junction
Protein 1 Antibody
NBP1-85047
Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody
Immunocytochemistry/ Immunofluorescence Tight Junction Protein 1 Antibody
Immunohistochemistry-Paraffin Tight Junction Protein 1 Antibody
(5 Reviews)
(20 Publications)
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Radixin Antibody
(1D9)
H00005962-M06
Western Blot Radixin Antibody (1D9)
Immunohistochemistry Radixin Antibody (1D9)
Immunocytochemistry/ Immunofluorescence Radixin Antibody (1D9)
(7 Publications)
Species: Ch, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
Moesin Antibody
(MSN/491)
NBP2-32875
Flow Cytometry Moesin Antibody (MSN/491)
Immunocytochemistry/ Immunofluorescence Moesin Antibody (MSN/491)
Western Blot Moesin Antibody (MSN/491)
(1 Publication)
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
PTRH1 Antibody
NBP1-86713
Immunohistochemistry-Paraffin PTRH1 Antibody
Species: Hu
Applications: IHC, IHC-P
PSD-95 Antibody
(6G6-1C9) - BSA Free
NB300-556
Flow Cytometry PSD-95 Antibody (6G6-1C9) - BSA Free
Immunocytochemistry/ Immunofluorescence PSD-95 Antibody (6G6-1C9) - BSA Free
Immunocytochemistry/ Immunofluorescence PSD-95 Antibody (6G6-1C9) - BSA Free
(1 Review)
(31 Publications)
Species: Hu, In, Mu, Pm, Rt
Applications: B/N, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
PTH1R/PTHR1
Antibody (3D1.1)
NBP1-40067
Immunohistochemistry PTH1R/PTHR1 Antibody (3D1.1)
Immunohistochemistry PTH1R/PTHR1 Antibody (3D1.1)
Western Blot PTH1R/PTHR1 Antibody (3D1.1)
(4 Publications)
Species: Bv, Ca, Eq, Hu, Pm, Mu, Po, Pm
Applications: ICC/IF, IHC, IHC-P, WB
SLC34A1 Antibody
(10B1.3E9) - BSA Free
NBP2-42216
Flow Cytometry SLC34A1 Antibody (10B1.3E9) - BSA Free
Flow Cytometry SLC34A1 Antibody (10B1.3E9) - BSA Free
Flow Cytometry SLC34A1 Antibody (10B1.3E9) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
PAG1 Antibody
(504004) [Unconjugate
MAB5285
Simple Western PAG1 Antibody (504004) [Unconjugated]
Western Blot PAG1 Antibody (504004) [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
PRRT2 Antibody -
BSA Free
NBP2-82026
Western Blot PRRT2 Antibody - BSA Free
Immunohistochemistry PRRT2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PRRT2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

Publications for NHERF-1 Recombinant Protein (H00009368-P01) (0)

There are no publications for NHERF-1 Recombinant Protein (H00009368-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NHERF-1 Recombinant Protein (H00009368-P01) (0)

There are no reviews for NHERF-1 Recombinant Protein (H00009368-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NHERF-1 Recombinant Protein (H00009368-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NHERF-1 Products

Research Areas for NHERF-1 Recombinant Protein (H00009368-P01)

Find related products by research area.

Blogs on NHERF-1

There are no specific blogs for NHERF-1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NHERF-1 GST (N-Term) Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol NHERF1