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NHERF-1 Antibody (EBP-10) [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
EBP-10
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

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NHERF-1 Antibody (EBP-10) [HRP] Summary

Immunogen
Bacterially produced recombinant full-length human NHERF1.
Specificity
The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NHERF1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for NHERF-1 Antibody (EBP-10) [HRP]

  • EBP50NHERF-1
  • ezrin-radixin-moesin binding phosphoprotein-50
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF1
  • Na+/H+ exchange regulatory co-factor
  • NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • NHERFNPHLOP2
  • Regulatory cofactor of Na(+)/H(+) exchanger
  • sodium/hydrogen exchanger regulatory factor 1
  • Sodium-hydrogen exchanger regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Solute carrier family 9 isoform A3 regulatory factor 1

Background

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NHERF1