Home » NGFR/TNFRSF16/p75NTR » NGFR/TNFRSF16/p75NTR Antibodies » NGFR/TNFRSF16/p75NTR Antibody (NGFR5)
Novus Biologicals products are now on bio-techne.com

NGFR/TNFRSF16/p75NTR Antibody (NGFR5)

 Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).
Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).

Product Details

Summary
Reactivity Hu, Fe, Ma, Pm, Pm, Rb, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
NGFR5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47965AF350
Alexa Fluor 405
NBP2-47965AF405
Alexa Fluor 488
NBP2-47965AF488
Alexa Fluor 532
NBP2-47965AF532
Alexa Fluor 594
NBP2-47965AF594
Alexa Fluor 647
NBP2-47965AF647
Alexa Fluor 700
NBP2-47965AF700
Alexa Fluor 750
NBP2-47965AF750
Allophycocyanin
NBP2-47965APC
Allophycocyanin/Cy7
NBP2-47965APCCY7
Biotin
NBP2-47965B
CoraFluor 1
NBP2-47965CL1
DyLight 350
NBP2-47965UV
DyLight 405
NBP2-47965V
DyLight 488
NBP2-47965G
DyLight 550
NBP2-47965R
DyLight 594
NBP2-47965DL594
DyLight 650
NBP2-47965C
DyLight 680
NBP2-47965FR
DyLight 755
NBP2-47965IR
FITC
NBP2-47965F
HRP
NBP2-47965H
Janelia Fluor 525
NBP2-47965JF525
Janelia Fluor 549
NBP2-47965JF549
Janelia Fluor 585
NBP2-47965JF585
Janelia Fluor 635
NBP2-47965JF635
Janelia Fluor 646
NBP2-47965JF646
Janelia Fluor 669
NBP2-47965JF669
PE
NBP2-47965PE
PE/Atto594
NBP2-47965PEATT594
PE/Cy5.5
NBP2-47965PECY55
PE/Cy7
NBP2-47965PECY7
PerCP
NBP2-47965PCP
Unconjugated
NBP2-47965-0.1mg
mFluor Violet 450 SE
NBP2-47965MFV450
mFluor Violet 500 SE
NBP2-47965MFV500
mFluor Violet 610 SE
NBP2-47965MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-47965-0.1mg
Azide and BSA Free
NBP2-47965-0.1mg
IHC-Prediluted
NBP2-48295
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

NGFR/TNFRSF16/p75NTR Antibody (NGFR5) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47965)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
NGFR from A875 melanoma cells
Epitope
aa 1-160
Localization
Cell surface and cytoplasmic
Marker
Soft Tissue Tumor Marker
Specificity
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NGFR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for NGFR/TNFRSF16/p75NTR Antibody (NGFR5)

  • CD271 antigen
  • CD271
  • Gp80-LNGFR
  • member 16
  • nerve growth factor receptor
  • NGF R
  • NGF receptor
  • NGFR
  • p75 ICD
  • p75 NTR
  • TNFRSF16
  • TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
  • tumor necrosis factor receptor superfamily member 16

Background

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(44 Publications)
Species: Rt
Applications: IHC, WB
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(76 Publications)
Species: Hu
Applications: ELISA
LEDGF Antibody
(762815) [Unconjugate
MAB3468
Western Blot LEDGF Antibody (762815) [Unconjugated]
Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC, WB
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Neutralization Siglec-7/CD328 Antibody [Unconjugated]
(1 Review)
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(57 Publications)
Species: Hu
Applications: BA
NTS1/NTSR1
Antibody - BSA Free
NLS938
Immunohistochemistry-Paraffin NTS1/NTSR1 Antibody - BSA Free
Immunohistochemistry-Paraffin NTS1/NTSR1 Antibody - BSA Free
Immunohistochemistry-Paraffin NTS1/NTSR1 Antibody - BSA Free
(1 Review)
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
NT-3 [Unconjugated]
267-N3
NT-3 [Unconjugated]
(72 Publications)
Species: Hu
Applications: BA
tropomyosin-3
Antibody (2E4)
H00007170-M02
Sandwich ELISA tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
(2 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
TrkB Antibody
[Unconjugated]
AF1494
Immunohistochemistry TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
(12 Reviews)
(79 Publications)
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB
Choline
Acetyltransferase/ChA
NBP1-30052
Immunohistochemistry-Paraffin Choline Acetyltransferase/ChAT Antibody
Immunocytochemistry/ Immunofluorescence Choline Acetyltransferase/ChAT Antibody
Immunocytochemistry/ Immunofluorescence Choline Acetyltransferase/ChAT Antibody
(24 Publications)
Species: Ch, Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
TrkC Antibody
[Unconjugated]
AF1404
Western Blot TrkC Antibody [Unconjugated]
Simple Western TrkC Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence TrkC Antibody [Unconjugated]
(1 Review)
(66 Publications)
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
NT-4 [Unconjugated]
268-N4/CF
NT-4 [Unconjugated]
(9 Publications)
Species: Hu
Applications: BA

Publications for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874) (0)

There are no publications for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874) (0)

There are no reviews for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NGFR/TNFRSF16/p75NTR Products

Research Areas for NGFR/TNFRSF16/p75NTR Antibody (NBP2-44874)

Find related products by research area.

Blogs on NGFR/TNFRSF16/p75NTR

There are no specific blogs for NGFR/TNFRSF16/p75NTR, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NGFR/TNFRSF16/p75NTR Antibody (NGFR5) and receive a gift card or discount.


Bioinformatics

Gene Symbol NGFR
Uniprot