Neuropilin-1 Antibody (130604)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5‑7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization.

Showing Publications 1 - 3 of 3.

Publications using MAB5661	Applications	Species
GJ Prud'homme, Y Glinka, Z Lichner, GM Yousef Neuropilin-1 is a receptor for extracellular miRNA and AGO2/miRNA complexes and mediates the internalization of miRNAs that modulate cell function Oncotarget, 2016-10-18;7(42):68057-68071. 2016-10-18 [PMID: 27486976] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme. Development, 2014-09-01;141(17):3420-30. 2014-09-01 [PMID: 25139858] (IHC, Mouse)	IHC	Mouse
Acevedo LM, Barillas S, Weis SM, Gothert JR, Cheresh DA Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood, 2008-01-07;111(5):2674-80. 2008-01-07 [PMID: 18180379] (IHC-Fr, Neutralization, Mouse)	IHC-Fr, Neutralization	Mouse

Array MAB5661

• Neuropilin-1 Antibodies
• Neuropilin-1 Lysates
• Neuropilin-1 Protein
• Neuropilin-1 ELISAs
• Neuropilin-1 Proteins and Enzymes