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Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]

 (6 Publications)
 
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Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at ...read more
Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of Hippocampus using Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF4414-SP
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Neuronal Pentraxin R/NPTXR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pentraxin R/NPTXR
Ala24-Lys499
Accession # O95502
Specificity
Detects human Neuronal Pentraxin R/NPTXR in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NPTXR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4414 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]

  • Neuronal Pentraxin R
  • neuronal pentraxin receptor
  • NPR
  • NPTXR

Background

NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65 kDa, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21 aa transmembrane segment, and a 476 aa extracellular region with one Pentraxin domain (aa 295‑499). One alternate start site exists at Met12, and a 55 kDa soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Over aa 24‑499, human NPTXR is 87% aa identical to mouse NPTXR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuronal Pentraxin R/NPTXR Antibody (AF4414)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(1)
Western Blot
(3)
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Human
(4)
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Showing Publications 1 - 6 of 6.
Publications using AF4414 Applications Species
MF Xiao, SE Roh, J Zhou, CC Chien, BP Lucey, MT Craig, LN Hayes, JM Coughlin, FM Leweke, M Jia, D Xu, W Zhou, C Conover Ta, DB Arnold, M Staley, C Jiang, IM Reti, A Sawa, KA Pelkey, CJ McBain, A Savonenko, PF Worley A biomarker-authenticated model of schizophrenia implicating NPTX2 loss of function Science Advances, 2021-11-24;7(48):eabf6935. 2021-11-24 [PMID: 34818031] (Western Blot, Human) Western Blot Human
Emma L van der Ende, Meifang Xiao, Desheng Xu, Jackie M Poos, Jessica L Panman, Lize C Jiskoot et al. Neuronal pentraxin 2: a synapse-derived CSF biomarker in genetic frontotemporal dementia Journal of Neurology, Neurosurgery & Psychiatry 6/1/2020 [PMID: 32273328]
MF Xiao, D Xu, MT Craig, KA Pelkey, CC Chien, Y Shi, J Zhang, S Resnick, O Pletnikova, D Salmon, J Brewer, S Edland, J Wegiel, B Tycko, A Savonenko, RH Reeves, JC Troncoso, CJ McBain, D Galasko, PF Worley NPTX2 and cognitive dysfunction in Alzheimer's Disease Elife, 2017-03-23;6(0):. 2017-03-23 [PMID: 28440221] (Western Blot, Human) Western Blot Human
Sung-Jin Lee Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses J. Neurosci, 2016-12-16;0(0):. 2016-12-16 [PMID: 27986928] (Western Blot, Human) Western Blot Human
Tina Bilousova, Karen Taylor, Ana Emirzian, Raymond Gylys, Sally A. Frautschy, Gregory M. Cole et al. Parallel age-associated changes in brain and plasma neuronal pentraxin receptor levels in a transgenic APP/PS1 rat model of Alzheimer's disease Neurobiology of Disease 2/1/2015 [PMID: 25449907]
von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4. Cancer Res, 2014-06-24;74(17):4796-810. 2014-06-24 [PMID: 24962026] (IHC, Human) IHC Human

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Bioinformatics

Gene Symbol NPTXR
Uniprot