![Western Blot: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - EK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MME ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME 1:500).](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Western-Blot-NBP2-72941-img0003.jpg "Western Blot: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - EK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MME ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME 1:500).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | OTI3D11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human MME(NP_009218) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MME |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 85.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Neprilysin/CD10 Antibody (NBP2-72941)Find related products by research area.
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| Gene Symbol | MME |
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0009.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
![Flow Cytometry: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - analysis of living 293T cells transfected with MME overexpression plasmid .](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72941-img0002.jpg "Flow Cytometry: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - analysis of living 293T cells transfected with MME overexpression plasmid .")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0004.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0005.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0006.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0007.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72941-img0008.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (OTI3D11) - Azide and BSA Free [NBP2-72941] - Staining of Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.")
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