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Neprilysin/CD10 Antibody (CB-CALLA)

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Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (CB-CALLA) [NBP2-44869] - Immunofluorescence staining of Ramos cells using Neprilysin/CD10 Antibody (CB-CALLA) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei were stained with Red Dot.
Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (CB-CALLA) [NBP2-44869] - Immunofluorescence staining of Ramos cells using Neprilysin/CD10 Antibody (CB-CALLA) followed by goat anti-Mouse IgG conjugated ...read more
Flow Cytometry: Neprilysin/CD10 Antibody (CB-CALLA) [NBP2-44869] - Flow Cytometric Analysis of Ramos cells using Neprilysin/CD10 Antibody (CB-CALLA) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
CB-CALLA
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47961AF350
Alexa Fluor 405
NBP2-47961AF405
Alexa Fluor 488
NBP2-47961AF488
Alexa Fluor 532
NBP2-47961AF532
Alexa Fluor 594
NBP2-47961AF594
Alexa Fluor 647
NBP2-47961AF647
Alexa Fluor 700
NBP2-47961AF700
Alexa Fluor 750
NBP2-47961AF750
Allophycocyanin
NBP2-47961APC
Allophycocyanin/Cy7
NBP2-47961APCCY7
Biotin
NBP2-47961B-100ul
Biotin
NBP2-47961B
CoraFluor 1
NBP2-47961CL1
DyLight 350
NBP2-47961UV
DyLight 405
NBP2-47961V
DyLight 488
NBP2-47961G
DyLight 550
NBP2-47961R
DyLight 594
NBP2-47961DL594
DyLight 650
NBP2-47961C
DyLight 680
NBP2-47961FR
DyLight 755
NBP2-47961IR
FITC
NBP2-47961F
Janelia Fluor 525
NBP2-47961JF525
Janelia Fluor 549
NBP2-47961JF549
Janelia Fluor 585
NBP2-47961JF585
Janelia Fluor 635
NBP2-47961JF635
Janelia Fluor 646
NBP2-47961JF646
Janelia Fluor 669
NBP2-47961JF669
PE
NBP2-47961PE
PE/Atto594
NBP2-47961PEATT594
PE/Cy5.5
NBP2-47961PECY55
PE/Cy7
NBP2-47961PECY7
PerCP
NBP2-47961PCP
mFluor Violet 450 SE
NBP2-47961MFV450
mFluor Violet 500 SE
NBP2-47961MFV500
mFluor Violet 610 SE
NBP2-47961MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47961-0.1mg
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Neprilysin/CD10 Antibody (CB-CALLA) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47961)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human Neprilysin/CD10 (Uniprot: P08473)
Localization
Cell Surface
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MME
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Neprilysin/CD10 Antibody (CB-CALLA)

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MME
Uniprot