| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, mIF |
| Clone | 715820 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human Neprilysin/CD10 Tyr52-Trp750 Accession # P08473 |
| Specificity | Detects human Neprilysin/CD10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Neprilysin is observed, and no cross-reactivity with rmKell, recombinant human (rh) ECE-1, rhECE-2, or rhNeprilysin-2 is observed. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MME |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration. |
Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).
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| Publication using MAB11821 | Applications | Species |
|---|---|---|
| Polyhydroxycurcuminoids but not curcumin upregulate neprilysin and can be applied to the prevention of Alzheimer's disease Sci Rep, 2016-07-13;6(0):29760. 2016-07-13 [PMID: 27407064] (Western Blot, Human) | Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
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