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NCK1 Antibody (4R1N9)

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Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NCK1] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4R1N9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NCK1 Antibody (4R1N9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333). PFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERG
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NCK1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NCK1 Antibody (4R1N9)

  • cytoplasmic protein NCK1
  • MGC12668
  • NCK adaptor protein 1melanoma NCK protein
  • NCK1
  • nck-1
  • NCKalpha
  • NCKNCK tyrosine kinase
  • non-catalytic region of tyrosine kinase
  • SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NCK1