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NCBP1 Antibody

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Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining in human testis and pancreas tissues using anti-NCBP1 antibody. Corresponding NCBP1 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining in human testis and pancreas tissues using anti-NCBP1 antibody. Corresponding NCBP1 RNA-seq data are presented for the ...read more
Immunocytochemistry/ Immunofluorescence: NCBP1 Antibody [NBP2-33897] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33897] - Staining of human testis shows high expression.
Western Blot: NCBP1 Antibody [NBP2-33897] - Analysis in human cell line RH-30.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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NCBP1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPH
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCBP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NCBP1 Protein (NBP2-33897PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NCBP1 Antibody

  • CBP80nuclear cap binding protein subunit 1, 80kD
  • NCBP 80 kDa subunit
  • NCBPMGC2087
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap-binding protein subunit 1,80 kDa nuclear cap-binding protein
  • Sto1

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Array NBP2-33897

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Bioinformatics

Gene Symbol NCBP1
Uniprot