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Napsin A Antibody (10C4B8) - BSA Free

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Western Blot: Napsin-A Antibody (10C4B8) [NBP2-37355] - Western blot analysis using NAPSA mouse mAb against rat liver tissue lysate.
Immunohistochemistry: Napsin-A Antibody (10C4B8) [NBP2-37355] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NAPSA mouse mAb with DAB staining.
Western Blot: Napsin-A Antibody (10C4B8) [NBP2-37355] - Western blot analysis using NAPSA mAb against human NAPSA recombinant protein. (Expected MW is 40.9 kDa)
Western Blot: Napsin-A Antibody (10C4B8) [NBP2-37355] - Western blot analysis using NAPSA mAb against HEK293 (1) and NAPSA (AA: 20-158)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Napsin-A Antibody (10C4B8) [NBP2-37355] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NAPSA mouse mAb with DAB staining.
ELISA: Napsin-A Antibody (10C4B8) [NBP2-37355] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
10C4B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Napsin A Antibody (10C4B8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Napsin A (AA: 20-158) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NAPSA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Napsin A Antibody (10C4B8) - BSA Free

  • Asp 4
  • ASP4
  • Aspartyl protease 4
  • EC 3.4.23
  • EC 3.4.23.-
  • EC 3.4.23.15
  • EC 3.4.23.3
  • EC 3.4.23.5
  • KAP
  • Kdap
  • NAP1napsin-A
  • NAPA
  • NAPApronapsin A
  • NAPSA
  • napsin A aspartic peptidase
  • Napsin A
  • Napsin-1
  • SNAPA
  • TA01/TA02

Background

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NAPSA