![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0011.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 5F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | NADK (AAH01709, 1 a.a. ~ 446 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG |
| Specificity | NADK - NAD kinase |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NADK |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Publication using H00065220-M01 | Applications | Species |
|---|---|---|
| Pollak N, Niere M, Ziegler M. NAD kinase levels control the NADPH concentration in human cells. J Biol Chem;282(46):33562-71. 2007-11-16 [PMID: 17855339] |
Secondary Antibodies |
Isotype Controls |
Research Areas for NADK Antibody (H00065220-M01)Find related products by research area.
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![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0008.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in HepG2.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0009.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in HepG2.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in Raw 264.7.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0010.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in Raw 264.7.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0012.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.")
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