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Recombinant Human Myosin Light Chain 2 His Protein

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SDS-Page: Recombinant Human Myosin Light Chain 2 Protein [NBC1-18536] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 20.9 kDa (186aa), confirmed by MALDI-TOF.Concentration: 0.5 mg/ml (determined by Bradford assay).
SDS-Page: Recombinant Human Myosin Light Chain 2 Protein [NBC1-18536] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 20.9 kDa (186aa), confirmed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human Myosin Light Chain 2 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids1-166 of Human Myosin Light Chain 2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MYL2
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBC1-18536.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 5mM CaCl2
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Myosin Light Chain 2 His Protein

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Myosin Light Chain 2 Recombinant Protein (NBC1-18536)(1)


Showing Publication 1 - 1 of 1.
Publication using NBC1-18536 Applications Species
Bandekar S Structure, Function, and Inhibition of the Oncogenic RhoGEF Trio Chem Biol 2009-04-25 [PMID: 19389625]

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Bioinformatics

Gene Symbol MYL2