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Human Myosin Light Chain 2 ELISA Kit (Colorimetric)

Name: Human Myosin Light Chain 2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39557

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ELISA: Human Myosin Light Chain 2 ELISA Kit (Colorimetric) [NBP3-39557] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.055 ng/mL (example only; lot dependent)

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Human Myosin Light Chain 2 ELISA Kit (Colorimetric) Summary

Description	Assay Length: 4.5 hours
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.055 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	MYL2

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Alternate Names for Human Myosin Light Chain 2 ELISA Kit (Colorimetric)

cardiac ventricular myosin light chain 2
CMH10myosin light chain 2
DKFZp779C0562
MLC2
MLC-2
MLC-2v
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
myosin, light chain 2, regulatory, cardiac, slow
myosin, light polypeptide 2, regulatory, cardiac, slow
regulatory light chain of myosin
RLC of myosin
slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Phospho-Specific

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Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Myosin Heavy Chain Antibody (MF20) [Unconjugated]
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Myosin Light Chain 2 Antibody
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Bioinformatics

Gene Symbol

MYL2

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human Myosin Light Chain 2 ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human Myosin Light Chain 2 ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human Myosin Light Chain 2 ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Myosin Light Chain 2 ELISA Kit (NBP3-39557) (0)

Reviews for Myosin Light Chain 2 ELISA Kit (NBP3-39557) (0)

Product General Protocols

FAQs for Myosin Light Chain 2 ELISA Kit (NBP3-39557) (0)

Additional Myosin Light Chain 2 Products

Research Areas for Myosin Light Chain 2 ELISA Kit (NBP3-39557)

Blogs on Myosin Light Chain 2

Customers Who Bought This Also Bought

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Product PDFs

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Bioinformatics