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Myosin Light Chain 2 Antibody (3B9-B4)

Images

 
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of MYL2 transfected lysate using anti-MYL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYL2 MaxPab ...read more
Sandwich ELISA: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Detection limit for recombinant GST tagged MYL2 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
3B9-B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Myosin Light Chain 2 Antibody (3B9-B4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MYL2 (AAH15821.1, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD
Specificity
MYL2 - myosin, light polypeptide 2, regulatory, cardiac, slow
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Publications
Read Publication using H00004633-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Myosin Light Chain 2 Antibody (3B9-B4)

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Light Chain 2 Antibody (H00004633-M01)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYL2
Entrez
OMIM
Uniprot