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Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

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Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635] Summary

Immunogen
Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myosin Light Chain 2 Antibody (NBP1-30249JF635) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYL2