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Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

Name: Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor&#174; 635]
Brand: Novus Biologicals
SKU: NBP1-30249JF635
Price: 499 USD

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Product Details

Summary

Reactivity	Hu, Mu, XpSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC
Clone	3B2
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 635

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-30249AF350

Alexa Fluor 405 NBP1-30249AF405

Alexa Fluor 488 NBP1-30249AF488

Alexa Fluor 532 NBP1-30249AF532

Alexa Fluor 594 NBP1-30249AF594

Alexa Fluor 647 NBP1-30249AF647

Alexa Fluor 700 NBP1-30249AF700

Alexa Fluor 750 NBP1-30249AF750

Allophycocyanin NBP1-30249APC

Allophycocyanin/Cy7 NBP1-30249APCCY7

Biotin NBP1-30249B

CoraFluor 1 NBP1-30249CL1

DyLight 350 NBP1-30249UV

DyLight 405 NBP1-30249V

DyLight 488 NBP1-30249G

DyLight 550 NBP1-30249R

DyLight 594 NBP1-30249DL594

DyLight 650 NBP1-30249C

DyLight 680 NBP1-30249FR

DyLight 755 NBP1-30249IR

FITC NBP1-30249F

HRP NBP1-30249H

Janelia Fluor 525 NBP1-30249JF525

Janelia Fluor 549 NBP1-30249JF549

Janelia Fluor 585 NBP1-30249JF585

Janelia Fluor 646 NBP1-30249JF646

Janelia Fluor 669 NBP1-30249JF669

PE NBP1-30249PE

PE/Atto594 NBP1-30249PEATT594

PE/Cy5.5 NBP1-30249PECY55

PE/Cy7 NBP1-30249PECY7

PerCP NBP1-30249PCP

Unconjugated NBP1-30249

mFluor Violet 450 SE NBP1-30249MFV450

mFluor Violet 500 SE NBP1-30249MFV500

mFluor Violet 610 SE NBP1-30249MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation NBP1-30249

BSA Free NBP1-30249

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-30249AF350
Alexa Fluor 405	NBP1-30249AF405
Alexa Fluor 488	NBP1-30249AF488
Alexa Fluor 532	NBP1-30249AF532
Alexa Fluor 594	NBP1-30249AF594
Alexa Fluor 647	NBP1-30249AF647
Alexa Fluor 700	NBP1-30249AF700
Alexa Fluor 750	NBP1-30249AF750
Allophycocyanin	NBP1-30249APC
Allophycocyanin/Cy7	NBP1-30249APCCY7
Biotin	NBP1-30249B
CoraFluor 1	NBP1-30249CL1
DyLight 350	NBP1-30249UV
DyLight 405	NBP1-30249V
DyLight 488	NBP1-30249G
DyLight 550	NBP1-30249R
DyLight 594	NBP1-30249DL594
DyLight 650	NBP1-30249C
DyLight 680	NBP1-30249FR
DyLight 755	NBP1-30249IR
FITC	NBP1-30249F
HRP	NBP1-30249H
Janelia Fluor 525	NBP1-30249JF525
Janelia Fluor 549	NBP1-30249JF549
Janelia Fluor 585	NBP1-30249JF585
Janelia Fluor 646	NBP1-30249JF646
Janelia Fluor 669	NBP1-30249JF669
PE	NBP1-30249PE
PE/Atto594	NBP1-30249PEATT594
PE/Cy5.5	NBP1-30249PECY55
PE/Cy7	NBP1-30249PECY7
PerCP	NBP1-30249PCP
Unconjugated	NBP1-30249
mFluor Violet 450 SE	NBP1-30249MFV450
mFluor Violet 500 SE	NBP1-30249MFV500
mFluor Violet 610 SE	NBP1-30249MFV610

Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635] Summary

Immunogen	Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).
Isotype	IgG2b Kappa
Clonality	Monoclonal
Host	Mouse
Gene	MYL2
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	ELISA Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

cardiac ventricular myosin light chain 2
CMH10myosin light chain 2
DKFZp779C0562
MLC2
MLC-2
MLC-2v
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
myosin, light chain 2, regulatory, cardiac, slow
myosin, light polypeptide 2, regulatory, cardiac, slow
regulatory light chain of myosin
RLC of myosin
slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Myosin Heavy Chain Antibody (MF20) [Unconjugated]
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Bioinformatics

Gene Symbol

MYL2

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

Species Glossary

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Product Details

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Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for Myosin Light Chain 2 Antibody (3B2) [Janelia Fluor® 635]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Myosin Light Chain 2 Antibody (NBP1-30249JF635) (0)

Reviews for Myosin Light Chain 2 Antibody (NBP1-30249JF635) (0)

Product General Protocols

Video Protocols

FAQs for Myosin Light Chain 2 Antibody (NBP1-30249JF635) (0)

Secondary Antibodies

Isotype Controls

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