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MYBBP1A Antibody

Images

 
Western Blot: MYBBP1A Antibody [NBP2-13630] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: MYBBP1A Antibody [NBP2-13630] - Staining of human cell line U-2 OS shows positivity in nucleoli and vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MYBBP1A Antibody [NBP2-13630] - Staining of human liver shows no positivity in nucleoli in hepatocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MYBBP1A Antibody [NBP2-13630] - Analysis in human skin and liver. Corresponding RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MYBBP1A Antibody [NBP2-13630] - Staining of human skin shows strong positivity in nucleoli in epidermal cells.
Immunohistochemistry-Paraffin: MYBBP1A Antibody [NBP2-13630] - Staining of human cerebral cortex shows strong positivity in nucleoli in neurons.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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MYBBP1A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: EDLPLCSILQQIQEKYDLHQVKKAMLRPALFANLFGVLALFQSGRLVKDQEALMKSVKLLQALAQYQNHLQEQPRKALVDILSEVSKATLQEILPEVLKADLNIILSSPEQLELFLLAQQKVPSKLKKLVG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYBBP1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
MYBBP1A Protein (NBP2-13630PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MYBBP1A Antibody

  • MYB binding protein (P160) 1a
  • myb-binding protein 1A
  • P160FLJ37886
  • p53-activated protein-2
  • PAP2

Background

Myb binding protein 1a (MYBBP1a) was originally isolated as a c-Myb interacting protein but has been found to associate with a number of transcription factors. MYBBP1a has been shown to activate or repress transcription through its interaction with transcriptional regulators such as AHR, jun, c-Myb, NF-kappaB, and PGC-1alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MYBBP1A