![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-44228-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).")
| Reactivity | RbSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Full length native Muscle Phosphofructokinase/PFKM/PFK-1 purified from rabbit muscle (Uniprot: P00511) |
| Specificity | Partial reactivity may occur against F6PK isolated from liver. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PFKM |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. |
| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
Research Areas for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (NBP2-44228)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44228-img0001.jpg "Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).")
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