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Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [DyLight 594]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 594

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Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [DyLight 594] Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 730 to 780 of human Phosphofructokinase, Muscle using the numbering given in entry NP_000280.1 (GeneID 5213).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFKM
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [DyLight 594]

  • 6-phosphofructokinase, muscle type
  • EC 2.7.1,6-phosphofructo-1-kinase
  • EC 2.7.1.11
  • GSD7
  • MGC8699
  • Muscle Phosphofructokinase
  • PFK1
  • PFK-1
  • PFKA
  • PFK-A
  • PFKM
  • PFKX
  • Phosphofructo-1-kinase isozyme A
  • Phosphofructokinase 1
  • phosphofructokinase, muscle
  • phosphofructokinase, polypeptide X
  • Phosphofructokinase-1
  • Phosphofructokinase-M
  • Phosphohexokinase

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PFKM