Home » Muscle Phosphofructokinase/PFKM/PFK-1 » PFK-1 Antibodies » Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [CoraFluor™ 1]
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Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Ca, EqSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-32169AF350
Alexa Fluor 405
NBP2-32169AF405
Alexa Fluor 488
NBP2-32169AF488
Alexa Fluor 532
NBP2-32169AF532
Alexa Fluor 594
NBP2-32169AF594
Alexa Fluor 647
NBP2-32169AF647
Alexa Fluor 700
NBP2-32169AF700
Alexa Fluor 750
NBP2-32169AF750
Allophycocyanin
NBP2-32169APC
Biotin
NBP2-32169B
DyLight 350
NBP2-32169UV
DyLight 405
NBP2-32169V
DyLight 488
NBP2-32169G
DyLight 550
NBP2-32169R
DyLight 594
NBP2-32169DL594
DyLight 650
NBP2-32169C
DyLight 680
NBP2-32169FR
DyLight 755
NBP2-32169IR
FITC
NBP2-32169F
HRP
NBP2-32169H
Janelia Fluor 525
NBP2-32169JF525
Janelia Fluor 549
NBP2-32169JF549
Janelia Fluor 646
NBP2-32169JF646
Janelia Fluor 669
NBP2-32169JF669
PE
NBP2-32169PE
PerCP
NBP2-32169PCP
Unconjugated
NBP2-32169
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Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Phosphofructokinase, Muscle using the numbering given in entry NP_000280.1 (GeneID 5213).
Predicted Species
Canine (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFKM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [CoraFluor™ 1]

  • 6-phosphofructokinase, muscle type
  • EC 2.7.1,6-phosphofructo-1-kinase
  • EC 2.7.1.11
  • GSD7
  • MGC8699
  • Muscle Phosphofructokinase
  • PFK1
  • PFK-1
  • PFKA
  • PFK-A
  • PFKM
  • PFKX
  • Phosphofructo-1-kinase isozyme A
  • Phosphofructokinase 1
  • phosphofructokinase, muscle
  • phosphofructokinase, polypeptide X
  • Phosphofructokinase-1
  • Phosphofructokinase-M
  • Phosphohexokinase

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PFKM