![Western Blot: MUPP1 Antibody [NBP1-49975] - Detection of Human MUPP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MUPP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MUPP1 was also immunoprecipitated by rabbit anti-MUPP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated MUPP1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/MUPP1-Antibody-Western-Blot-NBP1-49975-img0002.jpg "Western Blot: MUPP1 Antibody [NBP1-49975] - Detection of Human MUPP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MUPP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MUPP1 was also immunoprecipitated by rabbit anti-MUPP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated MUPP1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 1125 and 1150 of human Multiple PDZ Domain Protein using the numbering given in entry NP_003820.2 (GeneID 8777). |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MPDZ |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-49975 | Applications | Species |
|---|---|---|
| Lee CH, Yang JR, Chen CY et al. Novel STAT3 Inhibitor LDOC1 Targets Phospho-JAK2 for Degradation by Interacting with LNX1 and Regulates the Aggressiveness of Lung Cancer. Cancers (Basel) 2019-01-09 [PMID: 30634502] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MPDZ |
![Western Blot: MUPP1 Antibody [NBP1-49975] - LDOC1 interacts with Ligand Of Numb-Protein X 1 (LNX1) to promote phospho-JAK2 (pJAK2) degradation through the ubiquitin–proteasome pathway. (A,B) LDOC1 enhances proteasomal degradation (A) & ubiquitination (B) of pJAK2 in basal & IL-6-stimulated A549-derived cell lines. A549-derived cell lines were starved overnight & pretreated with CHX (100 μg/mL), either alone or with proteasome inhibitor MG132 (20 uM) for 3 h before IL-6 (100 ng/mL) stimulation. The cells were lysed in an NP-40 lysis buffer containing MG132 (20 μg/mL) & ubiquitin aldehyde (20 μg/mL) to inhibit isopeptidase activities. (A) Western blotting was performed to examine the levels of indicated proteins. (B,C) The cell extracts were precleared with mouse or rabbit IgG (IgGm or IgGr, respectively) before being immunoprecipitated using antibodies against either pJAK2 (B,C) or against LDOC1 & LNX1 antibodies (C). The input & the Immunoprecipitation (IP) fractions were analyzed through immunoblotting (blot) using antibodies that recognized the indicated proteins. After being stripped, the membrane was reblotted with an anti-pJAK2 antibody (B). (D) Associations between LDOC1-LNX1 & LDOC1-pJAK2 in IL-6-treated A549 & A549-shCtrl-D10 cells. IL-6 stimulated cells were subjected to immunofluorescence assay (IFA) by using anti-LDOC1-DyLight594 (red), anti-LNX1-FITC (green), & anti-pJAK2-FITC (green), respectively. The 4′,6-diamidino-2-phenylindole (DAPI, blue) was used for nuclear counterstaining. Images were captured using a confocal microscope. IP or IFA performed with IgGm or IgGr were used as negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30634502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-49975_rabbit-polyclonal-mupp1-antibody-310202416175282.jpg "Western Blot: MUPP1 Antibody [NBP1-49975] - LDOC1 interacts with Ligand Of Numb-Protein X 1 (LNX1) to promote phospho-JAK2 (pJAK2) degradation through the ubiquitin–proteasome pathway. (A,B) LDOC1 enhances proteasomal degradation (A) & ubiquitination (B) of pJAK2 in basal & IL-6-stimulated A549-derived cell lines. A549-derived cell lines were starved overnight & pretreated with CHX (100 μg/mL), either alone or with proteasome inhibitor MG132 (20 uM) for 3 h before IL-6 (100 ng/mL) stimulation. The cells were lysed in an NP-40 lysis buffer containing MG132 (20 μg/mL) & ubiquitin aldehyde (20 μg/mL) to inhibit isopeptidase activities. (A) Western blotting was performed to examine the levels of indicated proteins. (B,C) The cell extracts were precleared with mouse or rabbit IgG (IgGm or IgGr, respectively) before being immunoprecipitated using antibodies against either pJAK2 (B,C) or against LDOC1 & LNX1 antibodies (C). The input & the Immunoprecipitation (IP) fractions were analyzed through immunoblotting (blot) using antibodies that recognized the indicated proteins. After being stripped, the membrane was reblotted with an anti-pJAK2 antibody (B). (D) Associations between LDOC1-LNX1 & LDOC1-pJAK2 in IL-6-treated A549 & A549-shCtrl-D10 cells. IL-6 stimulated cells were subjected to immunofluorescence assay (IFA) by using anti-LDOC1-DyLight594 (red), anti-LNX1-FITC (green), & anti-pJAK2-FITC (green), respectively. The 4′,6-diamidino-2-phenylindole (DAPI, blue) was used for nuclear counterstaining. Images were captured using a confocal microscope. IP or IFA performed with IgGm or IgGr were used as negative controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30634502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")