![Western Blot: CRB3 Antibody [NBP1-98328] - Host: Rabbit. Target: CRB3. Positive control (+): HepG2 Cell Lysate (HG). Negative control (-): THP1 Cell Lysate (N30). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CRB3-Antibody-Western-Blot-NBP1-98328-img0003.jpg "Western Blot: CRB3 Antibody [NBP1-98328] - Host: Rabbit. Target: CRB3. Positive control (+): HepG2 Cell Lysate (HG). Negative control (-): THP1 Cell Lysate (N30). Antibody concentration: 1ug/ml")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to the N-terminal regeion of CRB3 Peptide sequence ANPGLGLLLALGLPFLLARWGRAWGQIQTTSANENSTVLPSSTSSSSDGN The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CRB3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 10 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
| Publication using NBP1-98328 | Applications | Species |
|---|---|---|
| Pan J, Liu F, Xiao X et al. METTL3 promotes colorectal carcinoma progression by regulating the m6A-CRB3-Hippo axis Journal of experimental & clinical cancer research : CR 2022-01-10 [PMID: 35012593] (WB) | WB |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: CRB3 Antibody [NBP1-98328] - Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell.](http://images.novusbio.com/fullsize/CRB3-Antibody-Western-Blot-NBP1-98328-img0002.jpg "Western Blot: CRB3 Antibody [NBP1-98328] - Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")