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MTIF2 Antibody - Azide and BSA Free

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Western Blot: MTIF2 Antibody [NBP2-95177] - Analysis of extracts of various cell lines, using MTIF2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody [NBP2-95177] - Analysis of U-2 OS cells using MTIF2 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human lung cancer using MTIF2 .
Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody [NBP2-95177] - Analysis of C6 cells using MTIF2 . Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody [NBP2-95177] - Analysis of L929 cells using MTIF2 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human colon carcinoma using MTIF2 .

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Catalog# & Formulation Size Price
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MTIF2 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2). GEEILEVESEPRAREVVDWRKYEQEQEKGQEDLKIIEEKRKEHKEAHQKAREKYGHLLWKKRSILRFLERKEQIPLKPKEKRERDSNVLSVIIKGDVDGSVEAILNIIDTYDASHECELELVHFGVGDVSANDVNLAETFDGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIYRLVEDLQEELSSRLPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLEKQKKFKLTRNGHVIWKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNMEFQVGDRIVCYEEKQIQAKTSWDPGF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTIF2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for MTIF2 Antibody - Azide and BSA Free

  • IF-2(Mt)
  • mitochondrial translational initiation factor 2
  • mitochondrial

Background

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MTIF2