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MRP2 Antibody (M2III-6)

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
M2III-6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

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MRP2 Antibody (M2III-6) Summary

Immunogen
202-amino acid sequence at C-terminal end of MRP2
Specificity
Reacts with an internal epitope of MRP2. Does not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ABCC2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Western Blot 1:20-1:50
Application Notes
M2III-6 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-6 (use 1:20) on acetone fixed frozen sections
Publications
Read Publication using
NBP1-42349 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID: 32697600).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for MRP2 Antibody (M2III-6)

  • ABC30
  • ABCC2
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 2
  • Canalicular multidrug resistance protein
  • canalicular multispecific organic anion transporter 1
  • CMOAT
  • CMOAT1
  • CMOATABC30
  • cMRP
  • DJS
  • KIAA1010
  • MRP2
  • MRP2ATP-binding cassette sub-family C member 2
  • Multidrug resistance-associated protein 2

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MRP2 Antibody (NBP1-42349)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-Fr.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ABCC2
Entrez
Uniprot