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Mitocondrial Translational Initiation Factor 3 Antibody

Images

 
Western Blot: Mitocondrial Translational Initiation Factor 3 Antibody [NBP1-92121] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Mitocondrial Translational Initiation Factor 3 Antibody [NBP1-92121] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: Mitocondrial Translational Initiation Factor 3 Antibody [NBP1-92121] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Mitocondrial Translational Initiation Factor 3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KHLVQITIKKGKNVDVSENEMEEIFHQILQTMPGIATFSSRPQAVQGGKALMCVLRALSKNEEKAYKETQETQERDTLNKDHGNDKESNVLHQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTIF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Mitocondrial Translational Initiation Factor 3 Protein (NBP1-92121PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Mitocondrial Translational Initiation Factor 3 Antibody

  • FLJ33676
  • IF3(mt)
  • IF-3mt
  • mitochondrial translational initiation factor 3
  • mitochondrial

Background

The Mitocondrial Translational Initiation Factor 3 gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism inthis gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcriptvariants. A pseudogene of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MTIF3