![Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-Azide-Free-Flow-Cytometry-NBP2-21789-img0001.jpg "Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, Flow, Func, IHC, IP, CyTOF-ready |
| Clone | M5/114 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Activated C57BL/6 mouse spleen cells |
| Specificity | The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | HLA-DMA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publications using NBP2-21789 | Applications | Species |
|---|---|---|
| Dharshika C, Gonzales J, Chow A et al. Stimulator of interferon genes (STING) expression in the enteric nervous system and contributions of glial STING in disease Neurogastroenterology & Motility 2023-02-27 (Immunohistochemistry) | Immunohistochemistry | |
| Chow AK, Grubisic V,Gulbransen BD Enteric glia regulate lymphocyte activation via autophagy-mediated MHC-II expression Cellular and molecular gastroenterology and hepatology 2021-06-21 [PMID: 34166814] (IF/IHC, Rat) | IF/IHC | Rat |
| Brown I The antioxidant glutathione protects against enteric neuron death in situ, but its depletion is protective during colitis. Am J Physiol Gastrointest Liver Physiol 2018-01-01 [PMID: 28882823] (IF/IHC, Mouse) | IF/IHC | Mouse |
| McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse) | FLOW | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for MHC Class II Antibody (NBP2-21789)Find related products by research area.
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Immune Cell Metabolic Flux Influences Type I Diabetes By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev... Read full blog post. |
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Read full blog post. |
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CD74 - a central player in antigen presentation by MHC class II Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in... Read full blog post. |
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Lymphocyte activation gene 3 (LAG3): No lag time in immune response The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required ... Read full blog post. |
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| Gene Symbol | HLA-DMA |
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