![Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- METTL3/MT-A70 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Methyltransferase-like-3-Antibody-Immunohistochemistry-Paraffin-NB100-77311-img0007.jpg "Immunohistochemistry-Paraffin: Methyltransferase like 3 Antibody [NB100-77311] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- METTL3/MT-A70 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339). |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | METTL3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Western_Blot_5819.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19720.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19954.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | METTL3 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311] - Detection of Human METTL3 by Western Blot of immunoprecipitants. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody (NB100-77311) used for IP at 3 mcg/mg lysate. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody (NB100-77310), which recognizes an upstream epitope. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.1 mcg/ml Detection: Chemiluminescence with exposure times of 30 seconds (A and B).anti-METTL3/MT-A70 antibody NB100-77310, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Methyltransferase-like-3-Antibody-Immunoprecipitation-NB100-77311-img0004.jpg "Immunoprecipitation: Methyltransferase like 3 Antibody [NB100-77311] - Detection of Human METTL3 by Western Blot of immunoprecipitants. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-METTL3/MT-A70 antibody (NB100-77311) used for IP at 3 mcg/mg lysate. METTL3/MT-A70 was also immunoprecipitated by rabbit anti-METTL3/MT-A70 antibody (NB100-77310), which recognizes an upstream epitope. For blotting immunoprecipitated METTL3/MT-A70, NB100-77310 was used at 0.1 mcg/ml Detection: Chemiluminescence with exposure times of 30 seconds (A and B).anti-METTL3/MT-A70 antibody NB100-77310, which recognizes an upstream epitope.")
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![Western Blot EMMPRIN/CD147 Antibody [Unconjugated]](https://images.novusbio.com/images2/EMMPRIN_AF972_Western_Blot_16707.jpg)
![Immunohistochemistry EMMPRIN/CD147 Antibody [Unconjugated]](https://images.novusbio.com/images2/EMMPRIN_AF972_Immunohistochemistry_17212.jpg)
![Knockout Validated EMMPRIN/CD147 Antibody [Unconjugated]](https://images.novusbio.com/images2/EMMPRIN_AF972_Knockout_Validated_22526.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![SDS-PAGE beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_321.jpg)
![Bioactivity beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_322.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")