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Mesothelin Antibody (420411) [Allophycocyanin]

 (5 Publications)
 (1 Review)
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HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin APC-conjugated Monoclonal Antibody (Catalog # FAB32652A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin APC-conjugated Monoclonal Antibody (Catalog # FAB32652A, filled histogram) or isotype control antibody (Catalog # IC006A, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
420411
Clonality
Monoclonal
Host
Rat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB32652U-100UG
Alexa Fluor 405
FAB32652V-100UG
Alexa Fluor 488
FAB32652G-100UG
Alexa Fluor 532
FAB32652X
Alexa Fluor 594
FAB32652T-100UG
Alexa Fluor 647
FAB32652R-100UG
Alexa Fluor 700
FAB32652N
Alexa Fluor 750
FAB32652S-100UG
Allophycocyanin/Cy7
FAB32652APCCY7
Biotin
FAB32652B
CoraFluor 1
FAB32652CL1
DyLight 350
FAB32652D
DyLight 405
FAB32652E
DyLight 488
FAB32652K
DyLight 550
FAB32652L
DyLight 594
FAB32652M
DyLight 650
FAB32652W
DyLight 680
FAB32652Y
DyLight 755
FAB32652Z
Fluorescein
FAB32652F
HRP
FAB32652H
Janelia Fluor 525
FAB32652JF525
Janelia Fluor 549
FAB32652I
Janelia Fluor 585
FAB32652JF585
Janelia Fluor 635
FAB32652JF635
Janelia Fluor 646
FAB32652J
Janelia Fluor 669
FAB32652JF669
PE/Atto594
FAB32652PEATT594
PE/Cy5.5
FAB32652PECY55
PE/Cy7
FAB32652PECY7
Phycoerythrin
FAB32652P
Unconjugated
MAB32652
mFluor Violet 450 SE
FAB32652MFV450
mFluor Violet 500 SE
FAB32652MFV500
mFluor Violet 610 SE
FAB32652MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB32652
Carrier Free
FAB32652F
Carrier Free
FAB32652P
Carrier Free
MAB32652
Carrier Free
FAB32652N
Carrier Free
MAB32652-SP
Carrier Free
FAB32652V-100UG
Carrier Free
FAB32652U-100UG
Carrier Free
FAB32652T-100UG
Carrier Free
FAB32652S-100UG
Carrier Free
FAB32652R-100UG
Carrier Free
FAB32652G-100UG
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Mesothelin Antibody (420411) [Allophycocyanin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272
Specificity
Detects human Mesothelin in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MSLN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
FAB32652A in the following application:
Publications
Read Publications using
FAB32652A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mesothelin Antibody (420411) [Allophycocyanin]

  • CAK1 antigen
  • CAK1
  • megakaryocyte potentiating factor
  • Mesothelin
  • MPF
  • MPFSMRP
  • MSLN
  • Pre-pro-megakaryocyte-potentiating factor
  • SMR
  • soluble MPF mesothelin related protein

Background

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes Megakaryocyte-Potentiating Factor (MPF). Mature human Mesothelin shares 60% amino acid identity with either mouse or rat Mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000902-M01
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Mesothelin
Antibody (420411) [Al ...
FAB32652A
Mesothelin Antibody (420411) [Allophycocyanin]
Species: Hu
Applications: Flow

Publications for Mesothelin Antibody (FAB32652A)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(3)
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Filter By Species
Human
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using FAB32652A Applications Species
Lee, D;Dunn, ZS;Guo, W;Rosenthal, CJ;Penn, NE;Yu, Y;Zhou, K;Li, Z;Ma, F;Li, M;Song, TC;Cen, X;Li, YR;Zhou, JJ;Pellegrini, M;Wang, P;Yang, L; Unlocking the potential of allogeneic V?2 T cells for ovarian cancer therapy through CD16 biomarker selection and CAR/IL-15 engineering Nature communications 2023-11-08 [PMID: 37938576] (Flow Cytometry, Human) Flow Cytometry Human
L Sommerfeld, I Knuth, F Finkernage, J Pesek, WA Nockher, JM Jansen, U Wagner, A Nist, T Stiewe, S Müller-Brü, R Müller, S Reinartz Prostacyclin Released by Cancer-Associated Fibroblasts Promotes Immunosuppressive and Pro-Metastatic Macrophage Polarization in the Ovarian Cancer Microenvironment Cancers, 2022-12-13;14(24):. 2022-12-13 [PMID: 36551640] (Flow Cytometry, Human) Flow Cytometry Human
Esther Schoutrop, Stefanie Renken, Isabella Micallef Nilsson, Paula Hahn, Thomas Poiret, Rolf Kiessling et al. Trogocytosis and fratricide killing impede MSLN-directed CAR T cell functionality OncoImmunology 2022-12-31 [PMID: 35898704]
Lin Ye, Yuqing Lou, Liming Lu, Xiaohong Fan Mesothelin‑targeted second generation CAR‑T cells inhibit growth of mesothelin‑expressing tumors in�vivo Experimental and Therapeutic Medicine 2018-11-27 [PMID: 30651858]
Servais EL, Suzuki K, Colovos C An in vivo platform for tumor biomarker assessment. PLoS ONE, 2011-10-26;6(10):e26722. 2011-10-26 [PMID: 22046338] (Flow Cytometry, Human) Flow Cytometry Human

Review for Mesothelin Antibody (FAB32652A) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using FAB32652A:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 Flow Human 01/23/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22498111
SpeciesHuman

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FAQs for Mesothelin Antibody (FAB32652A) (0)

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Secondary Antibodies

 

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 Verified Customer
01/23/2015
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol MSLN
Uniprot